Медико-биологический
информационный портал
для специалистов
 
БИОМЕДИЦИНСКИЙ ЖУРНАЛ Medline.ru

СОДЕРЖАНИЕ ЖУРНАЛА:
Физико-химическая биология

Клиническая медицина

Профилактическая медицина

Медико-биологические науки


АРХИВ:

Фундаментальные исследования

Организация здравохраниения

История медицины и биологии



Последние публикации

Поиск публикаций

Articles

Архив :  2000 г.  2001 г.  2002 г. 
               2003 г.  2004 г.  2005 г. 
               2006 г.  2007 г.  2008 г. 
               2009 г.  2010 г.  2011 г. 
               2012 г.  2013 г.  2014 г. 
               2015 г.  2016 г.  2017 г. 
               2018 г.  2019 г.  2020 г.  2021 г. 

Редакционная информация:
        Опубликовать статью
        Наша статистика


 РЕДАКЦИЯ:
Главный редактор

Заместители главного редактора

Члены редколлегии
Специализированные редколлегии


 УЧРЕДИТЕЛИ:
Институт теоретической и экспериментальной биофизики Российской академии наук.

ООО "ИЦ КОМКОН".




Адрес редакции и реквизиты

199406, Санкт-Петербург, ул.Гаванская, д. 49, корп.2

ISSN 1999-6314

Российская поисковая система
Искать: 


«
Vol. 21, Art. 6 (pp. 55-74)    |    2020       
»

Types of pulmonary artery injuries, nervous structures and environmental fat cell in the pig bifurction area in circular radio frequency ablation
Almazov National Medical Research Centre



Brief summary

Pulmonary arterial hypertension leads to right ventricular failure and premature death. Recently, a new method for treating the disease has been proposed - radiofrequency ablation, designed to destroy sympathetic nerve fibers and ganglia in adventitia and perivascular fatty tissue of the pulmonary artery. Purpose. To evaluate the various types and degree of damage to the wall of the pulmonary artery, nerve fibers and surrounding fatty tissue during radiofrequency ablation in a pig experiment. Materials and methods. In 17 Landrace pigs, radiofrequency ablation of the pulmonary artery was performed. In 9 cases, ablation was carried out with the aim of creating a mark on the intimate (group No. 1), in 8 cases, with the aim of creating transmural necrosis of the wall (group No. 2). A histological and immunohistochemical study was performed with antibodies to S100, tyrosine hydroxylase, muscarinic acetylcholine receptor M1, dopamine receptor D5. Results. A morphological study revealed the presence of dissections, hemorrhages in media and adventitia, coagulation of adventitia and adipose tissue in both groups, but the length of the latter was greater in group No. 2. Only in 2 cases was necrosis of a part of the nervous structures recorded (in group No. 1 and No. 2). There was no complete correspondence between macroscopic, histological and immunohistochemical changes. The latter were more pronounced. The largest area of expression loss was observed at the M1-muscarinic acetylcholinergic receptor. Conclusion. The most sensitive method for determining morphological damage during ablation of the pulmonary artery is immunohistochemical analysis, and the most sensitive marker is the M1 receptor. Insignificant damage to the nerve structures in our experiment may be due to the fact that the process of their fibrous replacement is stretched over time, which is caused not by direct, but by ischemic-mediated damage.


Key words

pulmonary hypertension; pulmonary artery denervation; radiofrequency ablation





(The article in PDF format. For preview need Adobe Acrobat Reader)



Open article in new window

Reference list

1. Chen S.L., Zhang Y.J., Zhou L., et al. Percutaneous pulmonary artery denervation completely abolishes experimental pulmonary arterial hypertension in vivo. EuroIntervention. 2013;9:269-276. doi: 10.4244/EIJV9I2A43.


2. Garcia-Lunar I., Pereda D., Santiago E., et al. Effect of pulmonary artery denervation in postcapillary pulmonary hypertension: results of a randomized controlled translational study. Basic Research in Cardiology. 2019;114(2). doi:10.1007/s00395-018-0714-x


3. Zhang H., Chen S.L. Pulmonary Artery Denervation: Update on Clinical Studies.Curr Cardiol Rep. 2019;21(10):124. doi: 10.1007/s11886-019-1203-z.


4. Rothman A.M., Arnold N.D., Chang W., et al. Pulmonary Artery Denervation Reduces Pulmonary Artery Pressure and Induces Histological Changes in an Acute Porcine Model of Pulmonary Hypertension. Circ Cardiovasc Interv. 2015;8:e002569. doi: 10.1161/CIRCINTERVENTIONS.115.002569


5. McMahon T.J., Hood J.S., Kadowitz P.J. Pulmonary vasodilator response to vagal stimulation is blocked by N omega-nitro-L-arginine methyl ester in the cat. Circ Res. 1992;70:364-9. doi: 10.1161/01.res.70.2.364


6. Rothman A.M., Jonas M., Caste D., et al. Pulmonary artery denervation using catheter-based ultrasonic energy. EuroIntervention. 2019;15:722-730. doi: 10.4244/EIJ-D-18-01082


7. Vaillancourt M., Chia P., Sarji S., et al. Autonomic nervous system involvement in pulmonary arterial hypertension. Respir Res. 2017;18(1):201.doi:10.1186/s12931-017-0679-6


8. Sakaoka A., Takami A., Onimura Y., et al. Acute changes in histopathology and intravascular imaging after catheter-based renal denervation in a porcine model. Catheterization and Cardiovascular Interventions. 2017;90(4), 631-638. doi:10.1002/ccd.27158


9. Su E., Zhao L., Gao C., et al. Acute changes in morphology and renal vascular relaxation function after renal denervation using temperature-controlled radiofrequency catheter. BMC Cardiovasc Disord. 2019;19(1):67. doi:10.1186/s12872-019-1053-z


10. Taborsky M., Richter D., Tonar Z., et al. Early morphologic alterations in renal artery wall and renal nerves in response to catheter-based renal denervation procedure in sheep: difference between single-point and multiple-point ablation catheters. Physiol Res. 2017;66:601-614. doi: 10.33549/physiolres.933503


11. Steigerwald K., Titova A., Malle C., et al. Morphological assessment of renal arteries after radiofrequency catheter-based sympathetic denervation in a porcine model. Journal of Hypertension. 2012;30(11), 2230-2239. doi:10.1097/hjh.0b013e32835821e5


12. Taborsky M., Richter D., Tonar Z., et al. Evaluation of Later Morphologic Alterations in Renal Artery Wall and Renal Nerves in Response to Catheter-Based Renal Denervation in Sheep: Comparison of the Single-Point and Multiple-Point Ablation Catheters. Physiol Res. 2018;67(6):891-901. doi:10.33549/physiolres.933903





Свидетельство о регистрации сетевого электронного научного издания N 077 от 29.11.2006
Журнал основан 16 ноября 2000г.
Выдано Министерством РФ по делам печати, телерадиовещания и средств массовых коммуникаций
(c) Перепечатка материалов сайта Medline.Ru возможна только с письменного разрешения редакции

Размещение рекламы

Rambler's Top100