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Vol. 21, Art. 20 (pp. 227-235)    |    2020       

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Early changes in extracellular dna and other parameters in the peripheral blood during irradiation of the bladder

1SBI «N.N. Petrov National Medical Research Center of Oncology» of the Ministry of Healthcare of the Russian Federation
2Granov Russian Scientific Center of Radiology and Surgical Technologies of Ministry of Healthcare of the Russian Federation

Brief summary

On the model of radiation cystitis, the changes in the number of blood cells, extracellular DNA (exDNA) and some indicators of total blood metabolism in serum early after the local exposure of the pelvic area of female Wistar rats at a dose of 25 Gy were studied. The irradiation of the region of the bladder of rats was carried out on a linear electron accelerator. 6 h after the exposure, a decrease in the absolute number of leukocytes, lymphocytes, monocytes were observed, and 24 h - a decrease of granulocytes. At 6 and 24 hours after irradiation, a significant increase in serum exDNA content was observed with normalization after 48 h. Irradiation led to a significant decrease in serum triglycerides content after 24 h after exposure, without changing other studied metabolic parameters. According to the correlation analysis, the relative change in the content of exDNA (0 h vs 6 h) correlated with changes in triglycerides (0 h vs 24 h), in the absence of significant correlations with a decrease in the number of blood cells.


Key words

local radiation, radiation cystitis, blood cells, extracellular DNA, triglycerides.

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