Опубликовать статью

Информационное партнерство

Инфоресурсы

Контакты

«

Vol. 24, Art. 91 (pp. 1344-1356)    |    2023       

»

Method for measuring benzo[a]pyrene metabolite concentrations in human urine by gc/ms for the purpose of exposure biomonitoring

Blinkov A.A., Semenov S.Y., Novikov S.V.

Federal State Unitary Enterprise Research and Technical Center of Radiation - Chemical Safety and Hygiene of the Federal Medical - Biological Agency, Moscow, Russian Federation

Brief summary

The authors developed and tested method for measuring (MM) the metabolite of benzo[a]pyrene (BaP) -3-hydroxybenzo[a]pyrene (3-OH-BaP) in urine. Since benzo[a]pyrene is one of the most carcinogenic polycyclic aromatic hydrocarbons (PAHs), the measurement of its metabolites will provide the most accurate assessment of PAH exposure by employees of enterprises, including those working in especially hazardous working conditions, serviced by the Federal Medical and Biological Agency of Russia. The technique is based on hydrolysis of urine samples, extraction, concentration and derivatization with N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA) followed by analysis of the sample using a combination of gas chromatography with high resolution mass spectrometry (GC-HRMS). For more confident identification and accurate determination of trace levels of 3-hydroxybenzo[a]pyrene in urine, an internal standard method and a double focusing magnetic sector mass spectrometer were used. The MM of 3-hydroxybenzo[a]pyrene was tested on a formed cohort of ten volunteers. As a result, the metrological characteristics of MM were determined and metrological certification was carried out.


Key words

3-hydroxybenzo[a]pyrene; PAH metabolites; biomonitoring; biomarker; high-resolution gas chromatography-mass spectrometry (GC-HRMS); MSTFA derivatization

Reference list

1. Seo S.H., Jung K.S., Park M.K., et al. Indoor air pollution of polycyclic aromatic hydrocarbons emitted by computers. Building and Environment. 2022; 218.


2. Nazar W., Niedoszytko M., Tchounwou P. Air Pollution in Poland: A 2022 Narrative Review with Focus on Respiratory Diseases. Int J Environ Res Public Health. 2022; 19(2): 895; doi: 10.3390/ijerph19020895.


3. Cerniglia C.E. Biodegradation of polycyclic aromatic hydrocarbons. Bioderedation. 1992; Vol.3: S 351-368.


4. Blumer M., Blumer W., Reich T. Polycyclic aromatic hydrocarbons in soils of a mountain valley: Correlation with highway traffic and cancer incidence. Environmental Science and Technology. 1977; N.11, S.1082-1084.


5. Li P.H., Wang Y., Li Y.H., et al. Gas-particle partitioning and precipitation scavenging of polycyclic aromatic hydrocarbons (PAHs) in the free troposphere in southern China. Atmospheric Environment. 2016; N128: S. 165-174.


6. Ren C., Wu Y., Zhang S., et al. PAHs in sediment cores at main river estuaries of Chaohu Lake: implication for the change of local anthropogenic activities. Environmental science and pollution research international. 2015; V.22, S.1687-1696.


7. Albuquerque M., Coutinho M., Borrego C. Long-term monitoring and seasonal analysis of polycyclic aromatic hydrocarbons (PAHs) measured over a decade in the ambient air of Porto, Portugal. Science of The Total Environmen. 2016; N.543(Part A), S.439-448.


8. Stading R., Gastelum G., Chu C., et al. Molecular Mechanisms of Pulmonary Carcinogenesis by Polycyclic Aromatic Hydrocarbons (PAHs): Implications for Human Lung Cancer. Semin Cancer Biol. 2021; N.76, S. 3-16.


9. Ravindra K., Sokhi R., Grieken R. Atmospheric polycyclic aromatic hydrocarbons: sourceattribution, emission factors and regulation. Atmos Environ. 2015; N. 42, S.2895-921.


10. Shimada T., Fujii-Kuriyama Y. Metabolic activation of polycyclic aromatic hydrocarbons to carcinogens by cytochromes P450 1A1 and 1B1. Cancer Sci. 2004; S1-6.


11. Szulejko J.E., Kim K.H., Brown R.J., Bae M.S. Review of progress in solvent-extraction techniques for the determination of polyaromatic hydrocarbons as airborne pollutants. TrAC Trends in Analytical Chemistry. 2014; N. 61, S. 40-48.


12. Talaska G., Thoroman J., Schuman B., Käfferlein H.U. Biomarkers of polycyclic aromatic hydrocarbon exposure in European coke oven workers. Toxicology Letters. 2014; N. 231, S. 213-216.


13. Rykovodstvo po ekspertize lekarstvennih sredstv. T.1, s.201.


14. Luo K., Gao Q., Hu J. Derivatization method for sensitive determination of3-hydroxybenzo[a]pyrene in human urine by liquidchromatography-electrospray tandem mass spectrometry. Journal of Chromatography A. 2015; S. 51-55.


15. Ariese F., Verkaik M., Hoornweg G.P., et al. Trace Analysis of 3-Hydroxybenzo[a]pyrene in Urine for the Biomonitoring of Human Exposure to Polycyclic Aromatic Hydrocarbons. Journal of Analytical Toxicology. 1994; V.18.