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Vol. 24, Art. 27 (pp. 371-379)    |    2023       

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Antioxidant defence enzymatic component?s deficiency and its prognostic impact on rat death evolution in acute inhalation severe poisoning with a pulmonotoxicant

Kon`shakov Yu.O., Ustinova T.M., Gusak T.I., Vengerovich N.G.

State Scientific Research Testing Institute of Military Medicine of the Ministry of Defense of the Russian Federation

Brief summary

Purpose: to study the dynamic pattern in glutathione peroxidase activity in the acute phase of perfluoroisobutylene poisoning at a toxic dose of LCt50 in experiment, and to substantiate the death probability prediction model in accordance with the obtained results. Methods: modeling of inhalation poisoning with perfluoroisobutylene was carried out by static seeding for 10 minutes at a toxic dose of 1 LCt50 (1,46?0,21 mg×min/l). Glutathione peroxidase was evaluated 30, 60, 120 and 360 minutes post inhalation exposure. The enzyme was studied using a spectrophotometric technique based on determining the loss value of reduced glutathione in the incubation medium during the reduction of glutathione peroxidase hydroperoxides. The simple logistic regression technique was used in the prediction model development. The non-parametric method of correlation analysis with Kendall rank correlation was used to confirm a relationship between the glutathione peroxidase activity level and the animals? death. Results: studies have shown a correlation between low levels of glutathione peroxidase and the subsequent animals? death. The relationship was confirmed between 87,5% of the indicator pairs being compared. A mathematical rat death prediction model was developed using the antioxidant enzyme laboratory value in the acute phase of perfluoroisobutylene poisoning as a predictor. Conclusion: there was a significant decrease in blood glutathione peroxidase activity was found in acute inhalation poisoning with severe perfluoroisobutylene. A high (more than 80%) correlation between the antioxidant enzyme glutathione peroxidase?s concentration decrease and the subsequent death of poisoned animals has been proven.


Key words

perfluoroisobutylene; glutathione peroxidase activity; static inhalation; logistic regression; pulmonotoxicant; pulmonary edema

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