Опубликовать статью

Информационное партнерство

Инфоресурсы

Контакты

«

Vol. 24, Art. 23 (pp. 325-336)    |    2023       

»

Evaluation of the toxic effects of p-chlorobenzophenone on the hepatorenal system in a subacute experiment

Larisa G. Gorokhova1,2, Tatyana K. Yadykina1

1FSBSI ?Research Institute for Complex Problems of Hygiene and Occupational Diseases?, Novokuznetsk
2Kuzbass Humanitarian Pedagogical Institute of FSBEI HE ?Kemerovo State University?, Novokuznetsk

Brief summary

The problem of the prevalence of chronic diseases of the hepatorenal system of chemical etiology is becoming increasingly important, due to the production characteristics of the chemical industry. Toxicological assessment of the nature of the impact of substances on organs and organ systems is a key preventive measure to prevent intoxication of the body of workers with industrial xenobiotics. The aim of the study was an experimental study of the toxic effects of p-chlorobenzophenone on the hepatorenal system in a subacute experiment. Materials and methods. In experiments, the toxic effects of p-chlorobenzophenone on the hepatorenal system were studied. The studies were carried out on outbred white rats. Using standard biochemical and morphological parameters, the effect of the substance on experimental animals in the subacute experiment was assessed. Serum indicators were analyzed: activity of aspartate aminotransferase, alanine aminotransferase, concentration of total protein, urea, cholesterol, chloride ions, percentage of protein fractions. The content of protein and chloride ions was determined in the urine. Morphological studies were performed: the brain, heart, lungs, liver, kidneys, spleen, stomach, pancreas and thyroid glands. The obtained results were processed using the Statistic 10.0 software package. Results. Changes in blood biochemical parameters were revealed: an increase in the activity of aminotransferases, an increase in the concentration of total protein, cholesterol, and urea in the blood serum. In rats in the process of seeding, changes in indicators characterizing the functional state of the kidneys were noted - an increase in spontaneous water diuresis, a decrease in the relative density of urine. A decrease in the total protein content in the urine was noted with an increase in the content of chlorine ions. Histological data confirm the development of the toxic effect of p-chlorobenzophenone on the hepatorenal system of animals exposed to poisoning. Limitations of the study. The study is limited to the study of the effects of p-chlorobenzophenone in experimental animals with subacute intragastric administration. In accordance with the directive documents on the protection of experimental animals, the number of in vivo experiments is limited which is connected with the dangers of animals and with public ethical views on in vivo experiments. Conclusion. When p-chlorobenzophenone enters the body, there is a risk of developing pathology, primarily of the hepatobiliary and urinary systems. Long-term intake of a xenobiotic, even in small doses, into the body of people in contact with it, can cause clinically significant organ damage. The information obtained as a result of the toxico-hygienic study of the biological effects of p-chlorobenzophenone is the basis for the development of methods for the treatment and prevention of possible intoxications, can be useful for solving a wide range of tasks fixed in the control and supervisory activities of Rospotrebnadzor, hygienic regulation and monitoring and will contribute to prevention and reduction of the level of diseases associated with exposure to harmful factors in the working environment.


Key words

p-chlorobenzophenone; hepatotoxicity; nephrotoxicity

Reference list

1. Eselevich S.A., Badmaeva B.B., Kyrshakov V. S. Professionalnie toksicheskie zabolevaniya pecheni. V kn.: «Aktyalnie voprosi medicini tryda v ysloviyah novoi koronavirysnoi infekcii: Sbornik trydov zaochnoi naychno-prakticheskoi konferencii». Sankt-Peterbyrg: Severo-Zapadnii gosydarstvennii medicinskii yniversitet imeni I.I. Mechnikova; 2022: 75-92.


2. Ponamarev V.S., Lynegov A.M. Vozmojnosti prognosticheskoi toksikologii v ocenke potencialnoi gepatotoksichnosti lekarstvennih veshestv. Normativno-pravovoe regylirovanie v veterinarii. 2022; 1: 64-67. https://doi.org/10.52419/issn2782-6252.2022.1.64.


3. Haidarov M.M., Myradova R.R. Gepatotoksichnost lekarstvennih sredstv kak odna iz problem sovremennoi medicini. Nayka cherez prizmy vremeni. 2020; 11(44): 46-49.


4. Rahmanin U.A. Aktyalizaciya metodologicheskih problem reglamentirovaniya himicheskogo zagryazneniya okryjaushei sredi. Gigiena i sanitariya. 2016; 95(8): 701-707. https://doi.org/10.18821/0016-9900-2016-95-8-701-707.


5. Rahmanin U.A. Konceptyalnie i metodologicheskie aspekti gigieni kak osnovi razvitiya profilakticheskogo zdravoohraneniya. Rossiiskii jyrnal reabilitacionnoi medicini. 2017; (1): 57-78.


6. Meshakova N.M., Shayahmetov S.F., Dyakovich M.P. Sovershenstvovanie metodicheskih podhodov k ocenke riska naryshenii zdorovya y rabotaushih pri vozdeistvii himicheskogo faktora. Gigiena i sanitariya. 2017; 96(3): 270-4. https://doi.org/10.18821/0016-9900-2017-96-3-270-274.


7. Byhtiyarov I.V., Denisov E.I., Lagytina G.N. i dr. Kriterii i algoritmi ystanovleniya svyazi narysheniya zdorovya s rabotoi. Medicina tryda i promishlennaya ekologiya. 2018; (8): 4-12. https://doi.org/10.31089/1026-9428-2018-8-4-12.


8. Rysakov N.V. Metodologicheskie problemi neinfekcionnoi epidemiologii i gigieni pri himicheskom zagryaznenii okryjaushei sredi. Gigiena i sanitariya. 2016; 95(9): 797-800. https://doi.org/10.18821/0016-9900-2016-95-9-797-800.


9. Sosedova L.M. Eksperimentalnie modeli v sisteme himicheskoi bezopasnosti rabotaushih. V kn.: Zdorove naseleniya i okryjaushaya sreda: Materiali Vserossiiskoi konferencii. Irkytsk; 2017.


10. Karkishenko N.N. Klassicheskie i alternativnie modeli v lekarstvennoi toksikologii. Biomedicina. 2006; (4): 1-23.


11. Birukova N.P., Rysakov S.V., Napalkova V.V. Obshie principi doklinicheskoi ocenki bezopasnosti farmakologicheskih lekarstvennih sredstv dlya veterinarnogo primeneniya. Veterinarnii vrach. 2018; (1): 3-9.


12. Voloshyk O.N., Kopilchyk G.P., Bychkovskaya I.M. Aktivnost markernih fermentov pecheni pri toksicheskom gepatite v ysloviyah alimentarnoi deprivacii proteina. Eksperimentalnaya i klinicheskaya gastroenterologiya. 2014; (8): 96-100.


13. Galatenko A.G. Modelirovanie toksicheskogo porajeniya pecheni na melkih laboratornih jivotnih. Sovremennie aspekti sanatorno-kyrortnogo lecheniya i reabilitacii na etapah okazaniya medicinskoi pomoshi detskomy i vzroslomy naseleniu. 2017; (1): 158-70.


14. Bishevskii A.Sh. Galyan S.L., Tersenov O.A. Biohimicheskie sdvigi i ih ocenka v diagnostike patologicheskih sostoyanii. M.: Med. Kniga; 2002.


15. Pshenkina N. N. Sivorotochnii albymin: stryktyra i transportnaya fynkciya (obzor). Medlain.ry. 2011; (12): 1067-1091. URL: http://medline.ru/public/art/tom12/art87.html (Data obrasheniya 24.03.2023).


16. Grizynov U.A., Grinberg A.A., Stypin V.A. i dr. Informativnost pokazatelya «effektivnaya koncentraciya albymina» pri rasprostranennom peritonite: dannie mnogocentrovogo issledovaniya. Anesteziologiya i reanimatologiya. 2003; 6: 32-35.


17. Tussupbekova G.A., Tuleuhanov S.T., Ablaikhanova N. et al. Research of biochemical and hematological parameters of blood of Caspian seals. Vestnik Kazahskogo nacionalnogo medicinskogo yniversiteta. 2018; 2: 218-223.


18. Emelyanchik S.V., Zimatkin S.M. i dr. Markernie biohimicheskie pokazateli krovi v ocenke dinamiki podpechenochnogo holestaza y kris. Vestnik Grodnenskogo gosydarstvennogo yniversiteta imeni Yanki Kypali. Seriya 5. Ekonomika. Sociologiya. Biologiya. 2015; 3(202): 130-136.


19. Litvickii P.F. Narysheniya ionnogo obmena. Voprosi sovremennoi pediatrii. 2015; 14 (3): S. 349-357. https://doi.org/10.15690/vsp.v14i3.1370.


20. Lu M., Jiebisch G., Wang W. Natric oxde links the apical Na+ conductance in the rat cortical collecting ducts. The Journal of General Physiology.1997; 110 (6): 717-26.


21. Myhin N.A., Natochin U.V. Vvedenie v nefrologiu. M.: GEOTAR-Media; 2007.


22. Haraldsson B., Sörensson J. Why do we not all have proteinuria? An update of our current understanding of the glomerular barrier. News in physiological sciences: an international journal of physiology produced jointly by the International Union of Physiological Sciences and the American Physiological Society. 2004; 19: 7-10.


23. Christensen E.L., Gburek J. Protein reabsorption in renal proximal tubule-function and dysfunction in kidney pathophysiology. Pediatric nephrology (Berlin, Germany). 2004; 19(7): 14-21.


24. Eremina E. U. Lekarstvennie porajeniya pecheni. Poliklinika. 2020; 6: 34-40.


25. Sokova E.A., Arhipov V.V., Mazerkina I.A., Myslimova O.V. Nekotorie aspekti ocenki lekarstvennogo porajeniya pochek. Bezopasnost i risk farmakoterapii. 2020; 8 (3): 123-133. https://doi.org/10.30895/2312-7821-2020-8-3-123-133.