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Vol. 23, Art. 15 (pp. 230-245)    |    2022       

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The aspects of methanols toxic effect in the presence of an alcohol dehydrogenase inhibitor

Yudin M.A.1,2, Czoj D.V.2, Vengerovich N.G.2, Bogachyova A.S. 1, Ustinova T.M.2, By`kova K.A.2

1North-Western State Medical University named after I.I. Mechnikov
2State scientific-research test Institute of military medicine of Defense Ministry of the Russian Federation

Brief summary

The features of sublethal (½ LD16) and lethal (1 LD50) rat poisoning with methanol were studied during the course (3 dose-escalation regimens) of the reversible alcohol dehydrogenase (ADH) inhibitor fomepizole (4-methylpyrazole). In these groups, a significant increase in formaldehyde concentration was noted in the first 6 hours after intragastric administration of methyl alcohol - 15- and 55-fold, respectively. Animal lethality within 2-6 days is associated with a significant increase of formic acid concentration in the plasma by 5-11 times, compared to intact animals. During methanol intoxication, fomepizole, used in various dosing regimens, did not affect the level of formaldehyde, but significantly reduced the formic acid concentration by 1.5 and 2 times 2 days after methanol poisoning in sublethal and lethal doses. Fomepizole provided a partial recovery of the blood plasma total antioxidant status (AOA), according to the ability to bind a stable radical diphenylpicrylhydrazyl (DPPH) and to the inhibition of 2-thiobarbituric acid (2-TBA) oxidation.


Key words

poisoning, methanol, alcohol dehydrogenase, fomepizole, formaldehyde, formic acid, cytoprotection, antioxidant activity.

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