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Vol. 19, Art. 21 (pp. 281-306)    |    2018       

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Morphological study of telocytes in different parts of the normal adult human brain

Mitrofanova LB¹, Khazratov AO¹, Krasnoshlyk PV¹, Vorobyova OM¹, Gorshkov AN¹², Gulyaev DA¹

1 - Almazov National Medical Research Centre, St. Petersburg, 197143
2 - Smorodintsev Research Institute of Influenza, St. Petersburg, 197376

Brief summary

Introduction: Telocytes have anti-inflammatory, regenerative and pacemaker properties, form a 3D network in almost all organs and coordinate intercellular relationships through multiple different contacts and microvesicles. These cells were found in the dura mater and choroid plexus of the cerebral ventricles. Objective: morphological study of telocytes in various parts of the normal brain. Materials and methods: Different parts of the normal brain of the 53-year-old woman and the 2 men aged 64 and 67 were examined. Two fragments of the normal tissue of the cerebellar hemispheres and two fragments of the dura mater were studied also in 2 men aged 45 and 39 (surgical material of the perifocal zone of astrocytoma and glioblastoma). Methods included histological and immunohistochemical study with CD117, NeuroD1, NeuroD1/connexin43 and CD34/connexin43 cocktails; electron microscopy and immunocytochemistry with CD117; isolation of telocyte cultures; phase-contrast microscopy and confocal laser scanning microscopy of cultures. Results: CD117-positive cells were detected in the dura mater (up to 24.5% of all cells), ventricular plexus (up to 28% of all cells), cerebral cortex (up to 1%), subcortical nuclei (up to 77% in some zones), the oblongated marrow (up to 14%), the pons (up to 12%), the corpus callosum (up to 95%), the hippocampus (from 0.0001 to 90% in different layers), the cerebellum of the normal brain (6 to 90 % in different departments). Cells with telocyte characteristics expressing NeuroD1 were identified using ultrastructural analysis and confocal microscopy of isolated cultures. Conclusion: The detection of telocytes with expression of NeuroD1 in many parts of the brain may indicate the possible involvement of these cells in the development of the central nervous system. Further understanding of the functions and origin of telocytes, the study of the role of telocytes in the oncogenesis of brain tumors can lead to new, purposeful methods of treating not only neurovascular diseases but also neoplasms.


Key words

brain telocytes, immunohistochemical examination, electron microscopy and immunocytochemistry, isolation of cultures

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