Опубликовать статью

Информационное партнерство

Инфоресурсы

Контакты

«

Vol. 16, Art. 37 (pp. 383-392)    |    2015       

»

Assessment of DNA damage in peripheral blood cells of people occupationally exposed to heavy metals

Tomilin N.V., Petrov A. N., Filko O. A., Khrabrova A. V., Solovyeva N. E.,
Ivanova T. M., Shestova G. V., Sizova K. V., Rutkovsky G. V., Glushkov R. K.,Ivanenko A. A., Solovyev N. D.

«Institute of Toxicology» of Federal Medico-Biological Agency

Brief summary

The alkaline gel-electrophoresis and micronucleus assay showed that the participants of exposed group had higher DNA damage of leucocyte’s nuclei and higher micronuclei frequency into reticulocytes than control group. The whole blood concentration of manganese was increasing too. Blood manganese concentration was negative correlated with DNA damage. It seems that manganese protects nuclear DNA from damage. It seems that at low concentration manganese was involved in antioxidant activity but at high concentration it was capable of to induce DNA damage in human leucocytes. Evidently genotoxicity of welding fumes is determined by other metals (lead, cobalt, nickel or aluminium), which are in welding fumes. It might be their combined action.


Key words

genotoxicity, leucocytes, reticulocytes, alkaline gel-electrophoresis, micronucleus assay, manganese

Reference list

1. Norbury C. J., Zhivotovsky B. DNA damage-induced apoptosis // Oncogene – 2004 – Vol.23 – P. 2797-2808.


2. Petrov A. N., Tomilin N. V., Filko O. A., Hrabrova A. V., Soloveva N. E., Ivanova T. M., Shestova G. V., Sizova K. V., Chernyak T. F., Rytkovskii G. V., Glyshkov R. K., Ivanenko A. A.,Solovev N. D. Ocenka stepeni povrejdeniya yadernoi DNK v kletkah perifericheskoi krovi cheloveka s pomoshu shelochnogo elektroforeza edinichnih kletok pri medicinskih osmotrah rabotnikov, professionalno svyazannih s vozdeistviem toksichnih metallov // Metodicheskie rekomendacii FMBA Rossii MR 12-37, Moskva. – 2011. – 26 c.


3. Hayashi M., Morita T., Kodama Y., et al. The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides // Mutat. Res. – 1990. – Vol. 245 (4) – P. 245-249.


4. Glyshkov R. K., Ivanenko A. A., Ivanenko N. B, Rytkovskii G. V. Metodika vipolneniya izmerenii massovoi koncentracii marganca, aluminiya, hroma i titana v krovi i plazme krovi cheloveka atomno-absorbcionnim metodom // Metodicheskie ykazaniya po metodam kontrolya, MYK 4.1.046-08, Moskva. – 2008. – 15 c.


5. Botta C., Iarmarcovai G., Chaspoul F., et al. Assesment of occupational exposure to welding fumes by inductively coupled plasma-mass spectroscopy and by the alkaline Comet assay // Environ Mol Mutagen. – 2006. – Vol. 47(4) – P. 284-295.


6. Tomilin N. V., Petrov A. N., Filko O. A., Hrabrova A. V., Soloveva N. E., Rytkovskii G. V., Glyshkov R. K. , Solovev N. D..Issledovanie genotoksicheskogo deistviya marganca i kadmiya v leikocitah perifericheskoi krovi belih kris. Himicheskaya i biologicheskaya bezopasnost. Specialnii vipysk, posvyashennii Federalnoi celevoi programme «Nacionalnaya sistema himicheskoi i biologicheskoi bezopasnosti Rossiiskoi Federacii (2009-2014 godi)». FMBA. M. Kirillica, 2012. – S. 8-14.


7. Oberlay L. W. Mechanism of the tumor suppressive effect of MnSOD overexpression // Biomed. Pharmacother. – 2005. – Vol. 59(4). – ´P. 143-148.


8. Day B. J., Kariya C. A novel class of cytochrome P450 reductase redox cyclers: cationic manganoporphyrins // Toxicol. Sci. – 2005. – Vol. 85(1) – P. 713-719.