Опубликовать статью

Информационное партнерство

Инфоресурсы

Контакты

«

Vol. 16, Art. 103 (pp. 1171-1183)    |    2015       

»

Study the effect of weak combined magnetic fields on regeneration, neoblasts proliferation and expression of wound-induced genes in planarians

Ermakov A.M.1,2, Skavulyak A.N.1, Ermakova O.N.1

1Federal State Budget Organization of Science, Institute of Theoretical and Experimental
Biophysics, Russian Academy of Sciences, 142290, Pushchino, Russia, Institutskaya 3
2Inter-regional public institution "Institute of Engineering Physics" 142210, Serpukhov, Russia, B. Udarniy per., D. 1a.

Brief summary

In experimental studies, we have found the ability to change the regeneration rate of planarians using alternating magnetic fields tuned to parametric resonance for the ions Ca2 + and K + in accordance with the Lednev's theory. Acceleration of planarian regeneration under the influence of the "calcium" combined magnetic field associated with the activation of the expression of wound-inducible genes in differentiated and stem cells and an increase in mitotic activity of planarian neoblasts. Deceleration of planarian regeneration under the "potassium" combined magnetic field associated with a decrease in the expression of wound-inducible genes in differentiated and stem cells, and a decline in mitotic activity of neoblasts. Combined magnetic fields tuned to different target change differently transcription of various planarian genes, as well as subsequent mitotic activity of neoblasts and planarian regeneration.


Key words

weak combined magnetic fields, planarian regeneration, mitotic activity, expression of genes.

Reference list

1. Belova N.A., Ermakov A.M., A.V. Znobisheva, Srebnickaya L.K., Lednev V.V. (2010) Vliyanie kraine slabih peremennih magnitnih polei na regeneraciu planarii i gravitacionnyu reakciu rastenii. Biofizika., T. 55(4). S. 704-709.


2. Lednev V.V. (1996) Bioeffekti slabih kombinirovannih, postoyannih i peremennih magnitnih polei. Biofizika. 41(1): 224-232.


3. Lednev V.V., Srebnickaya L.K., Ilyasova E.N., Rojdestvenskaya Z.E., Klimov A.A., Belova N.A., Tiras H.P. (1996(a)) Magnitnii parametricheskii rezonans v biosistemah: eksperimentalnaya proverka predskazanii teorii s ispolzovaniem regeneriryushih planarii Dugesia tigrina v kachestve test-sistemi. Biofizika. 41(4): 815-825.


4. Lednev V.V. Srebnickaya L.K., Ilyasova E.N., Rojdestvenskaya Z.E., Klimov A.A., Tiras H.P. (1996(b)) Slaboe kombinirovannoe magnitnoe pole, nastroennoe na parametricheskii rezonans yadernih spinov atomov vodoroda, yvelichivaet proliferativnyu aktivnost neoblastov v regeneriryushih planariyah Dugesia tigrina. DAN SSSR. 348(6): 830-833.


5. Sheiman I. M., Tiras H. P., Balobanova E. F. (1989) Morfogeneticheskaya fynkciya neiropeptidov. Fiziol J SSSR. 75: 619-626.


6. Belova N.A., Ermakova O.N., Ermakov A.M., Rojdestvenskaya Z. Ye., Lednev V.V. (2007) The bioeffects of extremely weak alternating magnetic fields. The Enviromentalist, v. 27, N4, p. 411-416.


7. Belova NA, Acosta-Avalos D. (2015) The Effect of Extremely Low Frequency Alternating Magnetic Field on the Behavior of Animals in the Presence of the Geomagnetic Field. J Biophys. 2015:423838.


8. Ermakova O.N., Ermakov A.M., Tiras Kh P., Lednev V.V. (2009) Melatonin effect on the regeneration of the flatworm Girardia tigrina. Ontogenez. 40(6):466-469.


9. King R.S., Newmark P.A. (2013) In situ hybridization protocol for enhanced detection of gene expression in the planarian Schmidtea mediterranea. BMC developmental biology. 13(8).


10. Lednev V.V. (1994) Interference with the vibrational energy sublevels of ions bound in calcium-binding proteins as the basis for the interaction of weak magnetic fields with biological systems. In: Frey, A.H. (ed.), On the Nature of Electromagnetic Field Interactions with Biological Systems. R.G. Landes Co., Austin, TX: 59-72.


11. Lednev V.V., Ermakov A.M., Ermakova O.N., Rozhdestvenskaya Z.E., Srebnitskaya L.K., Tiras Kh.P. (2005) Modulation of the effect of pharmacological agents by weak and extremely weak alternating magnetic fields on a model of regeneration of the planarian Girardia tigrina. Biophysics. 50(Suppl. 1). P. S130-S133.


12. Liboff A.R. (1992) Interaction Mechanism of Low - Level Electromagnetic Fields and Living Systems. Eds. B. Norden, C. Ramel. Oxford: Oxford University Press.: 130-147.


13. Newmark P.A., Sanchez Alvarado A. (2000) Bromodeoxyuridine specifically labels the regenerative stem cells of planarians. Developmental biology. 220(2):142-153.


14. Pearson B.J., Eisenhoffer G.T., Gurley K.A., Rink J.C., Miller D.E., Sanchez Alvarado A. (2009) Formaldehyde-based whole-mount in situ hybridization method for planarians. Developmental dynamics: an official publication of the American Association of Anatomists. 238(2):443-450.


15. Sanchez Alvarado A. (2007) Stem cells and the Planarian Schmidtea mediterranea. Comptes rendus biologies. 330(6-7):498-503.


16. Sturn A., Quackenbush J., Trajanoski Z. (2002) Genesis: cluster analysis of microarray data. Bioinformatics. Vol 18, N 1. P 207-208.


17. Wenemoser D., Lapan S.W., Wilkinson A.W., Bell G.W., Reddien P.W. (2012) A molecular wound response program associated with regeneration initiation in planarians. Genes & Development. 26(9):988-1002.


18. Wenemoser D., Reddien P.W. (2010) Planarian regeneration involves distinct stem cell responses to wounds and tissue absence. Developmental biology. 344(2):979-991.