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Vol. 14, Art. 24 (pp. 255-265)    |    2013       

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Algorithm obtaining of the hematopoietic stem cells peripheral blood for autologous transplantation.

Stepanov A.A.1, Korotaev E.B.1, Bessmeltsev S.S.2, Rabinovich V.I.1, Astahova L.P.1, Ponomarev S.A.1

1 Ugra research Institute of cellular technologies with stem cell Bank, Khanty-Mansiysk, Russia str. Mira 127-B, tel./fax (3467) 32-87-46, e-mail: glagolmail@gmail.com

2 FGBU \"Russian Research Institute of Hematology and Transfusiology of the Federal Medical and Biological Agency\", Saint-Petersburg

Brief summary

The article presents the results of clinical test of the algorithm of prolongation of apheresis leu-kocytes for receiving the hematopoietic stem cells from peripheral blood. The success of apheresis for obtaining good doses of hematopoietic stem cells for transplantation depended from timely start of operation, the duration of the operation and its multiplicity. It is established that the laboratory monitoring of quantity of leukocytes in peripheral blood in during of mobilization of hematopoietic stem cells is not informative for definition of the moment for the start of apheresis leukocytes. We received quite enough doses of hematopoietic stem cells for transplantation in 90% of apheresis leukocytes when the concentrations of hematopoietic stem cells was been more than 10 cells/mkl in the peripheral blood. If concentrations of hematopoietic stem cells was been more than 32 cells/mkl – we have had enough dose for transplantation from an alone operation. Practical using of the algorithm will reduce the number of repeated operations of apheresis leukocytes for 38%, and it will reduce the duration of mobilize and dose of drug. As the results it will be improve the effect of obtaining of hematopoietic stem cells of peripheral blood for trans-plantation.


Key words

hematopoietic stem cell, transplantation, granulocyte colony-stimulating factor, apheresis

Reference list

1. Laboratornii monitoring kolichestva leikocitov v perifericheskoi krovi pacienta v hode mobilizacii ne mojet slyjit nadejnim kriteriem opredeleniya momenta dlya nachala operacii leikocitafereza.


2. Kolichestvo GSK v perifericheskoi krovi pacienta v hode mobilizacii v diapazone ot 10 do 32 GSK/mkl yavlyaetsya optimalnim dlya provedeniya leikocitafereza.


3. Razrabotannii algoritm obosnovaniya ypravlyaemoi taktiki sbora aytotransplantacionnogo materiala v konkretnih klinicheskih sityaciyah pozvolit izbejat povtornogo leikocitafereza, sokratit medikamentoznyu mobilizaciu i polychat dostatochnyu transplantacionnyu dozy GSK.





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