1Military medical academy of S.М. Kirov, St.-Petersburg, Department of Forensic Medicine
2Universitet information technologies, mechanics and optics, St.-Petersburg, Department of Materials Science and Nanotechnology
Brief summary
The paper presents results comparative studies of different methods of sample preparation of rat blood for atomic force microscopy of red blood cells. Demonstrated the results of different variants sample preparation of blood of precision studies, the most often described in the literature. It is shown that the best facilitating the investigation of not only the size of red blood cells (diameter, height and depth of the central depression disc), but nanoscale membrane structure (pores in the membrane of red blood cells) is the method of fixing the smear of blood by drying in the air.
Key words
blood smear, sample preparation, the size of red blood cells, atomic force microscopy, glutaraldehyde, ethanol.
1. Chen Y ., Cai J . Membrane deformation of unfixed erythrocytes in air with time lapse investigated by tapping mode atomic force microscopy // Micron. - 2005. - Vol. 37(4). - P.339- 346.
2. Strasser S., Zink A., Kada G., Hinterdorfer P., Peschel O., Heckl W.M., Nerlich A.G., Thalhammer S. Age determination of blood spots in forensic medicine by force spectroscopy // Forensic Sci Int. - 2007. - Vol. 170(1). - P.8-14.
3. Mozhanova A. A., Nurgazizov N. I., Bukharaev A. A. Local elastic properties of biological materials studied by SFM //. Proc. of the SPM-2003. – 2003, March 2–5. - Nizhni Novgorod. - P.266–267.
4. Takeuchi M., Miyamoto H., Sako Y., Komizu H., Kuzumi A. Structure of the erythrocyte membrane skeleton as observed by atomic force microscopy // Biophysical J. - 1998. - Vol.74. – P.2171–2183.
5. Hofmann U. G., Rotsch C., Parak W. J., Radmacher M. Investigating the cytoskeleton of chicken cardiocytes with the atomic force microscope // J. of Structural Biology. – 1997. - Vol. 119. – P.84–91.
6. Shroff S. G., Saner D. R., Lal R. Dynamic micromechanical properties of cultured rat atrial myocytes measured by atomic force microscopy //American J. of Physiology (Cell Physiology 38). – 1995. - Vol. 269. - P.286–292.
7. Kubo N., Hirota R., Okada T., Ohara T., Kawano M., Itoh K. Structural Analysis Using Atomic Force Microscopy of the Surface of Red Blood Cells to Detect Anti-Red Blood Cell Autoantibody // Laboratory Hematology. – 2003. - Vol.9. – P.47-49.
8. Hekele O., Goesselsberger C.G., Gebeshuber I.C. Nanodiagnostics performed on human red blood cells with atomic force microscopy // Materials Science and Technology. – 2008. - Vol. 24(9). - P.1162-1165.
9. Ji X.-L., Ma Y.-M., Yin T., Shen M.-S., Xu X., Guan W. Application of atomic force microscopy in blood research // World J. Gastroenterol. – 2005. - Vol.11(10). – P.1709-1711.
10. Shroff S. G., Saner D. R., Lal R. Dynamic micromechanical properties of cultured rat atrial myocytes measured by atomic force microscopy //American J. of Physiology (Cell Physiology 38). – 1995. - Vol.269. - P.286–292.
11. Liu F., Burgess J., Mizukami H., Ostafin A. Sample preparation and imaging of erythrocyte cytoskeleton with the atomic force microscopy // Cell. Biochem . Biophys . – 2003. - Vol.38(3). – P.251-270.