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Vol. 13, Art. 80 (pp. 954-966)    |    2012       

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Especially preparation of blood samples for study of morphological parameters and structure of erythrocyte membranes by atomic force microscopy

Belousova O.D.1, Tolmachyev I.A.1, Gaidash A.A.1,
Levichev V.V.2, Gykov M.V.2, Belousov I.S.1

1Military medical academy of S.М. Kirov, St.-Petersburg, Department of Forensic Medicine
2Universitet information technologies, mechanics and optics, St.-Petersburg, Department of Materials Science and Nanotechnology

Brief summary

The paper presents results comparative studies of different methods of sample preparation of rat blood for atomic force microscopy of red blood cells. Demonstrated the results of different variants sample preparation of blood of precision studies, the most often described in the literature. It is shown that the best facilitating the investigation of not only the size of red blood cells (diameter, height and depth of the central depression disc), but nanoscale membrane structure (pores in the membrane of red blood cells) is the method of fixing the smear of blood by drying in the air.


Key words

blood smear, sample preparation, the size of red blood cells, atomic force microscopy, glutaraldehyde, ethanol.

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