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Vol. 13, Art. 73 (pp. 871-880)    |    2012       

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Determination of uranium in urine by sector field icp-ms

Ivanenko N.B., Navolotskiy D.V., Ivanenko A.A., Solovyev N.D., Blazhennikova I.V.

Brief summary

A method of urinary uranium determination based on sector field inductively coupled plasma mass spectrometry (SF-ICP-MS) has been suggested. For U determination in urine, the necessity of microwave sample digestion was shown. Analytical parameters were optimized, for both sample pretreatment and SF-ICP-MS detection. After the fulfillment of optimization stage, analytical figures of merit were estimated. The linear regression coefficient of the resulting calibration curve (concentration range of 0.01 to 10 μg / L) was found to be 0.998. Detection limit (3σ, n = 9) was 2 ng/l. The accuracy of the results was validated by analyzing the reference material Seronorm™ Trace Elements Urine and spiked urine samples for the validation in the whole dynamic range. For uranium concentration range of 0.01 to 1 mg/l, relative standard deviation (R.S.D.) ranged from 5 to 10%. For concentrations above 1 mg / l , R.S.D. did not exceed 2-3%. Preclinical studies of pharmaceuticals were carried out using the designed method. The efficiency of uranium eliminating therapy was evaluated on the basis of urinary uranium measurement in outbred male rats.


Key words

Uranium, urine, ICP-MS, microwave digestion, pharmaceuticals

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