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Vol. 13, Art. 32 (pp. 392-398)    |    2012       

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The impact of T-2 toxin on tributirin hydrolysis and some lipid exchange parameters in white outbred rats

Skalich I.P.*, Zhukov V.E.,

Research Institute of Hygiene, Toxicology and Occupational Pathology of Federal Medical and Biological Agency of Russia

Brief summary

The objective was to study the impact of T-2 toxin on the hydrolysis of tributyrin ( in small intestine) in white outbred male rats and at the same time to assess certain blood parameters of lipid exchange in the test animals. Mycotoxin was administered intragastrically as a single lose in the range of 0,56 to 2,8 mg/kg. It was found out that the exposure to T-2 toxin resulted in inhibiting the initial stages of tributyrin hydrolysis accompanied by the accumulation of triglycerides in the small intestine mucosal homogenate and by reducing total cholesterol level and tributyrinase activity. At the same time, a decrease in triglycerides concentration together with the rise of β-lipoprotein, β-lipoprotein cholesterol and total cholesterol content were registered in the blood serum of the test animals. All the revealed alterations complied with the “dose – effect” dependence. A correlation dependence was established between the lowered activity of the initial stages of tributyrin hydrolysis and the serum concentration of triglycerides (r = 0.97 when t = 8.08). It was concluded that the abnormalities of lipid exchange induced by a single T-2 exposure occurred even at the stage of absorption.


Key words

T-2 toxin, tributyrin, small intestine, blood lipids.

Reference list

1. SanPiN 2.3.2.1078-01 «Gigienicheskie trebovaniya bezopasnosti i pishevoi cennosti pishevih prodyktov» (zaregistrirovano v Minuste RF 22 marta 2002 g., registracionnii №3326).


2. Valiyllin, L. R. Toksikologicheskaya ocenka sochetannogo vozdeistviya zearalenona i T-2 toksina na jivotnih i iziskanie profilakticheskih sredstv: avtoref. diss... kand. biologicheskih nayk. - Kazan, 2008.- 137 s.


3. Sokolovis, M. T-2-toxin incidence and toxicity in pouitry / M. Sokolovis [et al.] // Arh Hig Rada Toksikol.- 2008.- V.59.-P.43-52.


4. Ygolev, A.M. Issledovanie pishevaritelnogo trakta cheloveka / A.M. Ygolev, N.N. Timofeeva, N. Iezyitova. - L.: Nayka, 1969.- S.182-183.


5. Kliniko-biohimicheskaya laboratornaya diagnostika: Spravochnik v 2-h tomah /Pod red. V.S. Kamishnikova. - Minsk: Interpresservis, 2003.


6. GOST R 53434-2009 Principi nadlejashei laboratornoi praktiki. Vveden 2009- 12-2 - № 554-st.


7. Glanc, S. Mediko-biologicheskaya statistika. Per. s angl. / S.Glanc.- M.: Praktika, 1999. - 459 s.


8. Belenkii, M.L. Elementi kolichestvennoi ocenki farmakologicheskogo effekta / M.L.Belenkii.- L.: Med. literatyra.- 1963.-152s.


9. Lodvigov, E.U. Kombinirovannoe vozdeistvie na jivotnih ioniziryushego izly- cheniya, karbofosa, T-2-toksina i iziskanie lechebno-profilakticheskih sredstv: av- toref. dis… kand. biol. nayk. – Kazan, 2011.- 21s.


10. Kalantari, H. Review on T-2-toxin / H. Kalantari, M. Moosavi // Jundishapur Journal of Natural Pharmfcentical Prodacts.- 2010.- № 5, is.1.- r.26-38.


11. Williams, Ph. P. Effects of T-2toxin on gastrointestinal tissues: a review of in vivo and in vitro models / Ph. P. Williams // Arch. Environ. Contam. and Toxicol. - 1989.- Vol. 18, №3.- R.374-387.


12. Judith, M. Determination of the Acute 50% Lethal Dose T-2 Toxin in Adult Bobwhite Quail: Additional Studies on the Effect of T-2 Mycotoxin on Blood Chemistry and the Morphology of Internal Organs / M. Judith, A. Grizzle, B.David // Avian Diseases.- 2003.- Vol. 48, № 2, R. 392–399.


13. Kyrashvili, L.V. Lipidnii obmen pri neotlojnih sostoyaniyah / L.V. Kyrashvili, V.G. Vasilkov. - Penza, 2003.- 234 s.


14. Voziyan, P.A. Stryktyrnaya organizaciya lipoproteinov plazmi krovi i ih vzaimodeistvie s kletkoi / P.A. Voziyan, U.D.Holodova // Ykr. biohim. jyrnj- 1989.-T.61, №3.-S.22-37.


15. Titov, V.N. Fyndamentalnaya medicina. Razlichie perenosa jirnih kislot v lipoproteinah ochen nizkoi plotnosti i nizkoi plotnosti i biologicheskie osnovi korrekcii semeinih giperlipoproteinemii / V.N. Titov // Klinicheskaya laboratornaya diagnostika. - 2005. - № 7. - S.3 -10.


16. Ahmadi, K. Effect of T-2 Toxin on Cell Death and Nitric Oxide Release by Mice Peritoneal / K. Ahmadi, M. Riazipoor // Macrophages Journal of Military medicine.- 2007.-V. 9, №1.- R.54-61


17. Baranec, A.A. Patogennoe deistvie mikotoksinov na organizm jivotnih i ego ocenka s pomoshu metodov in vitro / A.A.Baranec, G.A. Prigorelov //Aktyalnie problemi jivotnovodstva, veterinarnoi medicini, pererabotki selskohozyai- stvennoi prodykcii i tovarovedeniya: Materiali mejdynarodnoi naychno-prakti- cheskoi konferencii. – Voronej: FGOY VPO VGAY, 2010. –s. 158-161