Опубликовать статью

Информационное партнерство

Инфоресурсы

Контакты

«

Vol. 12, Art. 78 (pp. 936-947)    |    2011       

»

Real-time pcr platelet antigen-typing of donors and patients

Mineeva N.V., Yolov A.A., Burylev V.V., Gavrovskaya S.V., Chebotkevich V.N.

Brief summary

The real-time PCR – based test-system using the fluorescent probes on SNP determining the a- and b-alleles of platelet antigens HPA-1, -2, -3, -4, -5 and -15 is created and proposed. This system was tested on both blood donor samples and blood samples from parents with their children. The latest tests supported the efficiency of the proposed system for detection of mother-fetus conflicts for the children with thrombocytopenia symptoms. The results obtained with the proposed system are identical to the ones obtained with PROTRANS HPA Domino System (Germany).


Key words

platelet antigens, thrombocytopenia, fluorescent probes.

Reference list

1. Norton, A. Review: Platelet alloantigen and antibodies and their clinical significance/ A. Norton, D. Allen, M.Murphy//Immunohematology.-2004.-20.-v.2.-P.89-102.


2. Zotikov, E.A. Trombociti i antitrombocitarnie antitela/ E.A. Zotikov, A.G. Babaeva., L.L Golovkina. - M.: Monolit, 2003. – 128s.


3. Golovkina, L.L. Geneticheskii polimorfizm trombocitspecificheskih antigenov: avtoref. dis…. dokt. med. nayk.- M., 2008.-42s.


4. Cavanagh, G. HPA genotyping with sequence-specific priming (PCR-SSP): a streamlined method for rapid routine investigation/ G. Cavanagh, A. Dunn, C.E Chapman., A. Metcalfe// Transfusion Medicine.- 1997.- v.7.-№ 1.-R.41-45.


5. Allen, D. Survey of the use and clinical effectiveness of HPA-1a/5b-negative platelet concentrates in proven or suspected platelet alloimmunization/ D.Allen, J. Samon, S. Benjamin, et.al.//Transfusion Medicine.- 2004.-№14.-R.409-417.


6. Yolov, A.A. Genotipirovanie DNK donorov i recipientov krovi kak metod obespecheniya antigennoi bezopasnosti transfyzii/ A.A. Yolov, A.G. Lihonin // Transfyziologiya.- 2009.- t.10.- №1-2.-S.33.


7. Lucas, G. Recipient-derived HPA-1a antibodies: a cause of prolonged thrombocytopenia after unrelated donor stem cell transplantation/ G.Lucas, S. Culliford, F. Green, C. Sidra, A. Calvert, A. Green, P. Harrison, D. Harvey // Transfusion.- 2010.- 50.-R. 334-339.


8. Ghevaert, C. Developing recombinant HPA-1a–specific antibodies with abrogated Fcγ receptor binding for the treatment of fetomaternal alloimmune thrombocytopenia/ C.Ghevaert, D.A. Wilcox, J. Fang, K.L. Armour, M.K. Clark, W.H. Ouwehand, L.M. Williamson // J Clin Invest.- 2008.-№118(8).-R.2929–2938


9. Prenatal management of alloimmune thrombocytopenia of the fetus. International forum// Vox Sanguinis.- 2003.-v.84.-P. 142-149.


10. Ficko, T. Real-time PCR genotyping of human platelet alloantigens HPA-1, HPA-2, HPA- 3and HPA-5 is superior to the standard PCR-SSP method/T. Ficko, V. Galvani, R. Rupreht, T. Dovc, P. Rozman// Transfusion Medicine.- 2004.- v.14.- P.425–432.


11. Ruan, Li. Multicolor real-time polymerase chain reaction genotyping of six human platelet antigens using displacing probes/L.Ruan, P. Bin, L. Qingge// Transfusion.- 2007.- v.47.- P.1637- 1642.


12. Yolov, A.A. Tipirovanie antigenov trombocitov metodom PCR v realnom vremeni: Molekylyarnaya diagnostika – 2010/ A.A. Yolov, V.V Byrilyov., N.V. Mineeva, S.V. Gavrovskaya, V.N.Chebotkevich // Sb. tr. VII vseros. naych.-praktich. konf. s mejdynarod. ychastiem. –T. 3.- S.60-61.


13. Johnson, M.P. Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR/ M.P. Johnson, L.M. Haupt, L.R. Griffiths // Nucleic Acids Research .-2004.- v. 32.- No.6.-P. 55.


14. Mineeva, N.V. Metod viyavleniya antitel k antigenam trombocitov/N.V. Mineeva, M.N.Blinov, O.A. Zavarzina, E.V. Elhina, N.N. Bodrova, S.I. Kapystin, L.S. Priezjeva, I.V. Poedinenko //Transfyziologiya.- 2007.- №1-2.- S.47-48.


15. Salem, A.H. Allele frequencies of the human platelet antigen-1 in the Egyptian population/ A.H. Salem, K. Han, M.A. Batzer // BMC Research Notes.- 2009.- 2:90 doi:10.1186/1756-0500- 2-90


16. Krasnyakov, V.K. Polimorfizm genov trombocitov y donorov krovi Sankt- Peterbyrga/V.K. Krasnyakov, I. E. Pavlova, L. N. Bybnova //Vestnik Sankt-Peterbyrgskogo yniversiteta. -2009.-Ser.11.-Vip.2.- S.115-119.


17. Pavic, M. Gene frequencies of platelet-specific antigens in Croatian population/ M. Pavic, R. Zadro, M. Radic, S Dodig // Transfusion Medicine.- 2010.-v. 20.-№ 2.- P.73-77.