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Vol. 12, Art. 15 (pp. 179-184)    |    jan 2011       

№ гос. регистрации: 0421100002\0015

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Immunocytochemical studies cytological preparations liquid cytology of malignant pleuritis and ascitis

Belyaev A.M.³, Raskin G.A.^1,2, Zakharenko A.A.³, Kondratsov S.A.³

1 - City Clinical Oncology Dispensary, St. Petersburg,
2 - 122 Hospital, St. Petersburg,
3- Research Institute of Emergency Care. II Janelidze, St. Petersburg

Brief summary

Immunocytochemistry in its development fell far short of immunohistochemistry, which was due to inability to perform molecular studies on normal (conventional) smears. With the advent of liquid cytology, the problem ceased to exist. The most pressing issue for oncology, which can not be solved by other morphological methods, but cytological – it malignant pleuritis and ascitis. In the literature there are scattered reports on the decision point in the investigation of issues effusions, but no systematic approach to the diagnosis as in immunohistochemistry. The aim was to select panel of antibodies that effectively addresses the following issues: the differential diagnosis of the tumor with reactive changes, the diagnosis at a known location in history, the definition of the primary focus among the few without a known location. In this paper we have analyzed 30 malignant pleuritis and ascites . Selected panel of antibodies that solves our problem. Determination of the primary tumor without primary localization with immunocytochemistry requires further investigation.


Key words

liquid cytology; immunocytochemistry; pleuritis; ascitis.

Reference list

1. Binder R.A., Jencks J.A., Chun B., Rath C.E. "B" cell origin of malignant cells in a case of American Burkitt's lymphoma. Characterization of cells from a pleural effusion. // Cancer. - 1975 . – V.36(1). – P.161-8;


2. Lin O. Challenges in the interpretation of peritoneal cytologic specimens. // Arch Pathol Lab Med. – 2009. – V.33(5). – P. 739-42;


3. Davidson B., Risberg B., Kristensen G., Kvalheim G. et al. Detection of cancer cells in effusions from patients diagnosed with gynaecological malignancies. Evaluation of five epithelial markers. // Virchows Arch. – 1999. – V.435(1). – P.43-9;


4. Kim J.H., Kim G.E., Choi Y.D., Lee J.S. et al. Immunocytochemical panel for distinguishing between adenocarcinomas and reactive mesothelial cells in effusion cell blocks. // Diagn Cytopathol. – 2009. – V.37(4). – P.258-61;


5. Politi E., Kandaraki C., Apostolopoulou C., Kyritsi T., Koutselini H. Immunocytochemical panel for distinguishing between carcinoma and reactive mesothelial cells in body cavity fluids. // Diagn Cytopathol. – 2005. – V. 32(3). – P.151-5;


6. Park I.H., Kwon Y., Ro J.Y., Lee K.S., Ro J. Concordant HER2 status between metastatic breast cancer cells in CSF and primary breast cancer tissue. // Breast Cancer Res Treat. – 2009, [Epub ahead of print];


7. Han L., Pansare V., Al-Abbadi M. et al. Combination of MUC5ac and WT-1 immunohistochemistry is useful in distinguishing pancreatic ductal carcinoma from ovarian serous carcinoma in effusion cytology. // Diagn Cytopathol. – 2009. [Epub ahead of print];


8. Kobayashi M., Ueyama Y., Nakanishi H., Ishida H. et al. Immunocytochemical detection using CDX2. // Cancer Cytopathology. – V.108(2). – P. 114-118;


9. Lim B.J., Choi S.Y., Kang D.Y., Suh K.S. Peritoneal washing cytology of disseminated low grade endometrial stromal sarcoma: a case report. // Acta Cytol. – 2009.- V.53(5). – P. 587-90;


10. Li C., Ma Y., Xue Y., Zhang H., Wei Y. Molecular characterization and significance for prognosis of free gastric cancer cells in the peritoneal cavity. // Hepatogastroenterology. – 2009. – V.56(91-92). – P. 891-8.