Опубликовать статью

Информационное партнерство

Инфоресурсы

Контакты

«

Vol. 12, Art. 111 (pp. 1361-1371)    |    2011       

»

Influence of polyoxyethylene-polyoxypropylene based surfactants stabilizing the emulsion of perfluorocarbons, on the adsorption of human plasma proteins

Zhalimov V.K., Sclifas A.N., Kaptsov V.V., Kukushkin N.I.

Institute of Cell Biophysics, Russian Academy of Sciences

Brief summary

We studied the influence of the surfactants: proxanol 268, proxanol 168, Synperonic® F-108, Pluronic® P-123,- on the qualitative and quantitative adsorption of human plasma proteins on the surface of PFC emulsion. We found out that the maximum amount of protein (3.2 mg/ml) was adsorbed on the surface of the emulsion stabilized by proxanol 268; 2.05 and 0.75 mg/ml protein was adsorbed on the surface of the emulsion stabilized by proxanol 168 or Synperonic ® F-108, respectively; we observed the least amount of adsorbed protein (0.15 mg/ml) on the surface of the emulsion stabilized by Pluronic® P-123. The inverse relationship between the size of the hydrophobic part of surfactant molecules and the amount of adsorbed protein on the emulsion was shown. Significant differences in the qualitative composition of adsorbed proteins were observed for all the emulsions. At the same time it was established, that there was no adsorption of lipoproteins on the emulsion stabilized by Pluronic® P-123.


Key words

emulsion, protein adsorption, human plasma, protein, perfluorocarbons.

Reference list

1. Nanotehnologiya v blijaishem desyatiletii. Prognoz napravlenii issledovanii. Red. M. Roko, Z. Yilyams, P. Alivisatos. Mir, M, 2002.


2. Winter P.M., Cai K., Caruthers S.D. et al. // Expert. ReVol. Med. Devices., 2007, Vol. 4(2), P. 137–145.


3. Sklifas A.N., Shehtman D.G., Evdokimov V.A., Temnov A.A., Antashov A.V., Kapcov V.V., Kykyshkin N.I // Biofizika, 2002, t.47, vip.5, C. 926–932.


4. Sklifas A.N., Evdokimov V.A., Kykyshkin N.I. // Biofizika, 2008 ,tom 53, vip.2, C. 359–366.


5. Candiano G., Bruschi M., Musante L., et al., Electrophoresis, 2004, Vol. 25, P. 1327– 1333.