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Vol. 10, Art. 2 (pp. 27-43)    |    jan 2009       

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Novel cyto-bio - chemical method for revealing individual differencies of physiological states of the organism by complex character (pattern) of succinate dehydrogenase activity

Kondrashova M.N., Khunderyakova N.V., Zakharchenko M.V.

Institute of Theoretical and Experimental Biophysics RAS, Pushchino.

Brief summary

Novel method of succinate dehydrogenase (SDH) activity measurement in lymphocytes in smear of blood is elaborated. It is called: cyto-bio-chemical, because it combines the advantages of the standard cytochemical assay to study glass-adhered enzymes with that of modern biochemical methods for investigation of mitochondria. As a result, cytobiochemical method reveals in a smear of blood response of SDH to adrenaline in the organism which exceeds the level of quiescent state 200-400% and more instead of 20-40% activation determined by current methods. The important advantage of the method carried out by computer videomicroscopy of a great number of stained objects in a single smear is statistical validity of the each separate value obtained. The novel improvements make it possible to determine SDH activity more correctly and besides to reveal its different functional states. Novel tests are sensitive characters of individual differences in the state of the organism in norm, prepathology and diseases.


Key words

succinate dehydrogenase, cytochemistry, mitochondria, adrenaline, diagnostic set, lymphocytes

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